Membrane Proteins
Recommended Reading:
Pandey, Y., Dondapati, S. K., Wüstenhagen, D., Kubick, S. (2023): „Cell-Free Synthesis and Electrophysiological Analysis of Multipass Voltage-Gated Ion Channels Tethered in Microsomal Membranes“. Adv. Biochem. Eng. Biotechnol.
Ullrich, J., Ohlhoff, C., Dondapati, S. K., Zemella, A., Kubick, S. (2023): „Evaluation of the Ion Channel Assembly in a Eukaryotic Cell-Free System Focusing on Two-Pore Domain Potassium Channels K2P”. Int. J. Mol. Sci., 24, 6299
https://doi.org/10.3390/ijms24076299.
Pandey, Y., Dandapati, S. K., Kubick, S. (2023): „Enriched cell-free and cell-based native membrane derived vesicles (nMV) enabling rapid in-vitro electrophysiological analysis of the voltage-gated sodium channel 1.5”. Biochim. Biophys. Acta Biomembr. (BBA), 184144.
doi.org/10.1016/j.bbamem.2023.184144.
Haueis, L., Stech, M., Schneider, E., Lanz, T., Hebel, N., Zemella, A., Kubick, S. (2023): „Rapid One-Step Capturing of Native, Cell-Free Synthesized and Membrane-Embedded GLP-1R“. Int. J. Mol. Sci., 24, 2808.
Ullrich, J., Göhmann, P. J., Zemella, A., Kubick, S. (2022): „Oligomerization of the heteromeric γ-aminobutyric acid receptor GABAB in a eukaryotic cell-free system“. Sci. Rep., 12(1), 20742.
doi: 10.1038/s41598-022-24885-0.
Bazzone, A., Körner, A., Meincke, M., Bhatt, M., Dondapati, S., Barthmes, M., Kubick, S., Fertig, N. (2022): „SSM-based electrophysiology, a label-free real-time method reveals sugar binding & transport events in SGLT1”. Biosens. Bioelectron., 197, 113763.
doi: 10.1016/j.bios.2021.113763.
Dhandapani, P., Dondapati, S. K., Zemella, A., Bräuer, D., Wüstenhagen, D. A., Mergler, S., Kubick, S. (2021): „Targeted esterase-induced dye (TED) loading supports direct calcium imaging in eukaryotic cell-free systems”. RSC Adv., 11, 16285 – 16296.
Dondapati, S. K., Lübberding, H., Zemella, A., Thoring, L., Wüstenhagen, D. A., Kubick, S. (2019): „Functional Reconstitution of Membrane Proteins Derived From Eukaryotic Cell-Free Systems”. Front. Pharmacol., 10, 917.
doi: 10.3389/fphar.2019.00917.
Dondapati, S. K., Pietruschka, G., Thoring, L., Wüstenhagen, D. A., Kubick, S. (2019): „Cell-free synthesis of human toll-like receptor 9 (TLR9): Optimization of synthesis conditions and functional analysis”. PloS One, 14: e0215897.
doi: 10.1371/journal.pone.0215897.
Zemella, A., Richter, T., Thoring, L., Kubick, S. (2019): „A combined Cell-free Protein Synthesis and Fluorescence-Based Approach to Investigate GPCR Binding Properties”. Methods Mol. Biol., 1947, 57 – 77.
doi: 10.1007/978-1-4939-9121-1_4.g.
Dondapati, S. K., Wüstenhagen, D. A., Strauch, E., Kubick, S. (2018): „Cell-free production of pore forming toxins: Functional analysis of thermostable direct hemolysin from Vibrio parahaemolyticus”. Eng. Life Sci., 18, 140 – 148.
Dondapati, S. K., Wüstenhagen, D. A., Kubick, S. (2018): „Functional Analysis of Membrane Proteins Produced by Cell-Free Translation”. Methods Mol. Biol. (2018), 1685, 171 – 186.
doi: 10.1007/978-1-4939-7366-8_10.
Zemella, A., Grossmann, S., Sachse, R., Sonnabend, A., Schaefer, M., Kubick, S.: „Qualifying a eukaryotic cell-free system for fluorescence based GPCR analyses”. Sci. Rep. (2017), 7(1): 3740.
doi: 10.1038/s41598-017-03955-8.
Sonnabend, A., Spahn, V., Stech, M., Zemella, A., Stein, C., Kubick, S. (2017): „Production of G protein-coupled receptors in an insect-based cell-free system”. Biotechnol. Bioeng., 114, 2328 – 2338.
Zaitseva, E., del Rio Martinez, J., Dondapati, S. K., Baaken, G., Petersen, S., Kubick, S., Behrends, J. C (2016): „hERG Ion Channel Activity Measured in Planar Lipid Bilayer Arrays: Reconstitution from Eukaryotic Cell-Free Expression System and Cellular Membrane Preparations”. Biophysical Journal., 110(3), 446a.
doi: 10.1016/j.bpj.2015.11.2399.
Quast, R. B., Ballion, B., Stech, M., Sonnabend, A., Varga, B. R., Wüstenhagen, D. A., Kele, P., Schiller, S. M., Kubick, S. (2016): „Cell-free synthesis of functional human epidermal growth factor receptor: Investigation of ligand-independent dimerization in Sf21 microsomal membranes using non-canonical amino acids”. Sci. Rep., 6:34048.
Quast, R. B., Sonnabend, A., Stech, M., Wüstenhagen, D. A., Kubick, S. (2016): „High-yield cell-free synthesis of human EGFR by IRES-mediated protein translation in a continuous exchange cell-free reaction format”. Sci. Rep., 6:30399.
Dondapati, S. K., Kreir, M., Quast, R. B., Wüstenhagen, D. A., Brüggemann, A., Fertig, N., Kubick, S. (2014): „Membrane assembly of the functional KcsA potassium channel in a vesicle-based eukaryotic cell-free translation system”. Biosens. Bioelectron., 59, 174 – 183.
doi: 10.1016/j.bios.2014.03.004.
Sachse, R., Dondapati, S. K., Fenz, S. F., Schmidt, T., Kubick, S. (2014): „Membrane protein synthesis in cell-free systems: From bio-mimetic systems to bio-membranes”. FEBS Lett., 588, 2774 – 2781.
doi: 10.1016/j.febslet.2014.06.007.
Bechlars, S., Wüstenhagen, D. A., Drägert, K., Dieckmann, R., Strauch, E., Kubick, S. (2013): „Cell-free synthesis of functional thermostable direct hemolysins of Vibrio parahaemolyticus”. Toxicon, 76, 132 – 142.
doi: 10.1016/j.toxicon.2013.09.012.
Brödel, A. K., Sonnabend, A., Roberts, L. O., Stech, M., Wüstenhagen, D. A., Kubick, S. (2013): „IRES-mediated translation of glycoproteins and membrane proteins in eukaryotic cell-free systems”. PLoS One, 8(12):e82234.
doi: 10.1371/journal.pone.0082234.
Sachse, R., Wüstenhagen, D. A., Šamalíková, M., Gerrits, M., Bier, F. F., Kubick, S. (2013): „Synthesis of membrane proteins in eukaryotic cell-free systems”. Eng. Life Sci., 13(1), 39 – 48.
Zampatis, D. E., Rutz, C., Furkert, J., Schmidt, A., Wüstenhagen, D. A., Kubick, S., Tsopanoglou, N. E., Schülein, R. (2012): „The protease-activated receptor 1 possesses a functional and cleavable signal peptide which is necessary for receptor expression“. FEBS Lett., 586, 2351 – 2359.
doi: 10.1016/j.febslet.2012.05.042.
Kubick, S., Gerrits, M., Merk, H., Stiege, W., Erdmann, V. A. (2009): „In vitro Synthesis of posttranslationally modified Membrane Proteins”, Chapter 2. In: Membrane Protein Crystallization (Ed.: Larry DeLucas). Current Topics in Membranes, Vol. 63, Elsevier, 25 – 49.
doi: 10.1016/S1063-5823(09)63002-7.