Antibodies and Recombinant Immunotoxins
Recommended Reading:
Krebs, S. K., Stech, M., Jorde, F., Rakotoarinoro, N., Ramm, F., Marinoff, S., Bahrke, S., Danielczyk, A., Wüstenhagen, D. A., Kubick, S. (2022): „Synthesis of an Anti-CD7 Recombinant Immunotoxin Based on PE24 in CHO and E. coli Cell-Free Systems“. Int. J. Mol. Sci., 23 (22), 13697,
doi.org/10.3390/ijms232213697.
Haueis, L., Stech, M., Kubick, S. (2022): „A Cell-free Expression Pipeline for the Generation and Functional Characterization of Nanobodies“. Front. Bioeng. Biotechnol., 10:896763.
doi: 10.3389/fbioe.2022.896763.
Krebs, S. K., Rakotoarinoro, N., Stech, M., Zemella, A., Kubick, S. (2022): „A CHO Based Cell-Free Dual Fluorescence Reporter System for the Straightforward Assessment of Amber Suppression and scFv Functionality”. Front. Bioeng. Biotechnol., 10:873906.
doi: 10.3389/fbioe.2022.873906.
Stech, M., Rakotoarinoro, N., Teichmann, T., Zemella, A., Thoring, L., Kubick, S. (2021): „Synthesis of Fluorescently Labeled Antibodies Using Non-Canonical Amino Acids in Eukaryotic Cell-Free Systems”. In: Structural Proteomics. High-Throughput Methods (Ed.: Owens, R. J.). Part of the book series: Methods in Molecular Biology (MIMB), Vol. 2305, 175 – 190.
doi: 10.1007/978-1-0716-1406-8_9.
Stech, M., Nikolaeva, O., Thoring, L., Stöcklein, W. F. M., Wüstenhagen, D. A., Hust, M., Dübel, S., Kubick, S. (2017): „Cell-free synthesis of functional antibodies using a coupled in vitro transcription-translation system based on CHO cell lysates”. Sci. Rep., 7(1): 12030.
doi: 10.1038/s41598-017-12364-w.
Stech, M., Kubick, S. (2015): „Cell-free synthesis meets antibody production: A review”. Antibodies, 4, 12 – 33.
Stech, M., Hust, M., Schulze, C., Dübel, S., Kubick, S. (2014): „Cell-free eukaryotic systems for the production, engineering and modification of scFv antibody fragments”. Eng. Life Sci., 14, 387 – 398.
Stech, M., Merk, H., Schenk, J. A., Stöcklein, W., Wüstenhagen, D. A., Micheel, B., Duschl, C., Bier, F. F., Kubick, S. (2012): „Production of functional antibody fragments in a vesicle-based eukaryotic cell-free translation system”. J. Biotechnol., 164, 220 – 231.
doi: 10.1016/j.jbiotec.2012.08.020.