Building Blocks for Synthetic Biology
Recommended Reading:
Schloßhauer, J. L., Zemella, A., Dondapati, S. K., Thoring, L., Meyer, M., Kubick, S. (2023): „Enhancing the performance of a mutant pyrrolysyl‑tRNA synthetase to create a highly versatile eukaryotic cell‑free protein synthesis tool”. Sci. Rep., 13 (1), 15236.
doi: 10.1038/s41598-023-42198-8.
Pouyan, P., Zemella, A., Schloßhauer, J. L., Walter, R. M., Haag, R., Kubick, S. (2023): „One to one comparison of cell-free synthesized erythropoietin conjugates modified with linear polyglycerol and polyethylene glycol“. Sci. Rep., 13, 6394.
doi: 10.1038/s41598-023-33463-x.
Pandey, Y., Dandapati, S. K., Kubick, S. (2023): „Enriched cell-free and cell-based native membrane derived vesicles (nMV) enabling rapid in-vitro electrophysiological analysis of the voltage-gated sodium channel 1.5”. Biochim. Biophys. Acta Biomembr. (BBA), 184144.
doi.org/10.1016/j.bbamem.2023.184144.
Walter, R. M, Zemella, A., Schramm, M., Kiebist, J., Kubick, S. (2022): „Vesicle-based cell-free synthesis of short and long unspecific peroxygenases“. Front. Bioeng. Biotechnol., 10, 964396,
doi.org/10.3389/fbioe.2022.964396.
Knauer, J. F., Liers, C., Hahn, S., Wüstenhagen, D. A., Zemella, A., Kellner, H., Haueis, L., Hofrichter, M., Kubick, S. (2022): „Cell-free production of the bifunctional glycoside hydrolase GH78 from Xylaria polymorpha”. Enzyme and Microbial Technology, 161, 110110.
doi: 10.1016/j.enzmictec.2022.110110.
Ramm, F., Jack, L., Kaser, D., Schloßhauer, J. L., Zemella, A., Kubick, S. (2022): „Cell-Free Systems Enable the Production of AB5 Toxins for Diagnostic Applications”. Toxins, 14, 233.
Schloßhauer, J. L., Cavak, N., Zemella, A., Thoring, L., Kubick, S. (2022): „Cell Engineering and Cultivation of Chinese Hamster Ovary Cells for the Development of Orthogonal Eukaryotic Cell-free Translation Systems”. Front. Mol. Biosci., 9:832379.
doi: 10.3389/fmolb.2022.832379
Ramm, F., Dondapati, S. K., Trinh, H. A., Wenzel, D., Walter, R. M., Zemella, A. Kubick, S. (2022): „The Potential of Eukaryotic Cell-Free Systems as a Rapid Response to Novel Zoonotic Pathogens: Analysis of SARS-CoV-2 Viral Proteins”. Front. Bioeng. Biotechnol. (eCollection), 10:896751.
doi: 10.3389/fbioe.2022.896751.
Ramm, F., Stech, M., Zemella, A., Frentzel, H., Kubick, S. (2021): „The Pore‐Forming Hemolysin BL Enterotoxin from Bacillus cereus: Subunit Interactions in Cell‐Free Systems”. Toxins, 13, 807.
Wüstenhagen, D. A., Lukas, P., Müller, C., Aubele, S.A., Hildebrandt, J.-P., Kubick, S. (2020): „Cell-free Synthesis of the Hirudin Variant 1 of the blood-sucking Leech Hirudo medicinalis” Sci. Rep., 10:19818.
doi: 10.1038/s41598-020-76715-w.
Dondapati, S. K., Stech, M., Zemella, A., Kubick, S. (2020): „Cell‑Free Protein Synthesis: A Promising Option for Future Drug Development”. BioDrugs, 34, 327 – 348.
doi: 10.1007/s40259-020-00417-y.
Ramm, F., Dondapati, S. K., Thoring, L., Zemella, A., Wüstenhagen, D. A., Frentzel, H., Stech, M., Kubick, S. (2020): “Mammalian cell-free protein expression promotes the functional characterization of the tripartite non-hemolytic enterotoxin from Bacillus cereus”. Sci. Rep., 10:2887.
doi: 10.1038/s41598-020-59634-8.
Dondapati, S. K., Wüstenhagen, D. A., Strauch, E., Kubick, S. (2018): „Cell-free production of pore forming toxins: Functional analysis of thermostable direct hemolysin from Vibrio parahaemolyticus”. Eng. Life Sci., 18, 140 – 148.
Jérôme, V., Thoring, L., Salzig, D., Kubick, S., Freitag, R. (2017): „Comparison of cell-based vs. cell-free mammalian systems for the production of a recombinant human bone morphogenic growth factor”. Eng. Life Sci., 17, 1097 – 1107.
Quast, R. B., Ballion, B., Stech, M., Sonnabend, A., Varga, B. R., Wüstenhagen, D. A., Kele, P., Schiller, S. M., Kubick, S. (2016): „Cell-free synthesis of functional human epidermal growth factor receptor: Investigation of ligand-independent dimerization in Sf21 microsomal membranes using non-canonical amino acids”. Sci. Rep., 6:34048.
Georgi, V., Georgi, L., Blechert, M., Bergmeister, M., Zwanzig, M., Wüstenhagen, D. A., Bier, F. F., Jung, E., Kubick, S. (2016): „On-chip automation of cell-free protein synthesis: new opportunities due to a novel reaction mode”. LAB Chip., 16, 269 – 281.
Quast, R. B., Mrusek, D., Hoffmeister, C., Sonnabend, A., Kubick, S. (2015): „Cotranslational incorporation of non-standard amino acids using cell-free protein synthesis”. FEBS Lett., 589, 1703 – 1712.
doi: 10.1016/j.febslet.2015.04.041.
Fenz, S. F., Sachse, R., Schmidt, T., Kubick, S. (2014): „Cell-free synthesis of membrane proteins: tailored cell models out of microsomes”. Biochim. Biophys. Acta, 1838, 1382 – 1388.
doi: 10.1016/j.bbamem.2013.12.009.
Quast, R. B., Claußnitzer, I., Merk, H., Kubick, S., Gerrits, M. (2014): „Synthesis and site-directed fluorescence labeling of azido proteins using eukaryotic cell-free orthogonal translation systems“. Anal. Biochem., 451, 4 – 9.
doi: 10.1016/j.ab.2014.01.013.
Sachse, R., Dondapati, S. K., Fenz, S. F., Schmidt, T., Kubick, S. (2014): „Membrane protein synthesis in cell-free systems: From bio-mimetic systems to bio-membranes”. FEBS Lett., 588, 2774 – 2781.
doi: 10.1016/j.febslet.2014.06.007.
Scheller, F. W., Yarman, A., Bachmann, T., Hirsch, T., Kubick, S., Renneberg, R., Schumacher, S., Wollenberger, U., Teller, C., Bier, F. F. (2014): „Future of biosensors: a personal view”. Adv. Biochem. Eng. Biotechnol., 140, 1 – 28.
Stech, M., Brödel, A. K., Quast, R. B., Sachse, R., Kubick, S. (2013): „Cell-Free Systems: Functional Modules for Synthetic and Chemical Biology”. Adv. Biochem. Eng. Biotechnol., 137, 67 – 102.
Bechlars, S., Wüstenhagen, D. A., Drägert, K., Dieckmann, R., Strauch, E., Kubick, S. (2013): „Cell-free synthesis of functional thermostable direct hemolysins of Vibrio parahaemolyticus”. Toxicon, 76, 132 – 142.
doi: 10.1016/j.toxicon.2013.09.012.
Brödel, A. K., Raymond, J. A., Duman, J. G., Bier, F. F., Kubick, S. (2013): „Functional evaluation of candidate ice structuring proteins using cell-free expression systems”. J. Biotechnol, 163, 301 – 310.
doi: 10.1016/j.jbiotec.2012.11.001.
Brödel, A. K., Sonnabend, A., Roberts, L. O., Stech, M., Wüstenhagen, D. A., Kubick, S. (2013): „IRES-mediated translation of glycoproteins and membrane proteins in eukaryotic cell-free systems”. PLoS One, 8(12):e82234.
doi: 10.1371/journal.pone.0082234.
Shaklee, P. M., Semrau, S., Malkus, M., Kubick, S., Dogterom, M., Schmidt, T. (2010): „Protein Incorporation in Giant Unilamellar Vesicles under Physiological Conditions”. ChemBioChem, 11, 175 – 179.
Royall, E., Woolaway, K. E., Schacherl, I., Kubick, S., Belsham, G. J., Roberts, L. O. (2004): „The Rhopalosiphum padi virus 5' IRES is functional in Spodoptera frugiperda 21 cells and in their cell-free lysates; implications for the baculovirus expression system”. J. Gen. Virol., 85, 1565 – 1569.