Cell-free Systems
Recommended Reading:
Knauer, J. F., Schulz, C., Zemella, A., Wüstenhagen, D. A., Walter R. M., Küpper, J.-H., Kubick, S. (2024): „Synthesis of mono Cytochrome P450 in a modified CHO-CPR cell-free protein production platform”. Sci. Rep., 14, 1271, doi.org/10.1038/s41598-024-51781-6.
Dondapati, S. K., Stech, M., Zemella, A., Kubick, S. (2020): „Cell‑Free Protein Synthesis: A Promising Option for Future Drug Development”. BioDrugs, 34, 327 – 348.
doi: 10.1007/s40259-020-00417-y.
Thoring, L., Zemella, A., Wüstenhagen, D. A., Kubick, S. (2019): „Accelerating the Production of Druggable Targets: Eukaryotic Cell-free Systems Come into Focus”. Methods Protoc., 2, 30.
Zemella, A., Thoring, L., Hoffmeister, C., Šamalíková, M., Ehren, P., Wüstenhagen, D. A., Kubick, S. (2018): „Cell-free protein synthesis as a novel tool for directed glycoengineering of active erythropoietin”. Sci. Rep., 8(1): 8514.
doi: 10.1038/s41598-018-26936-x.
Thoring, L., Kubick, S. (2018): „Versatile Cell-Free Protein Synthesis Systems Based on Chinese Hamster Ovary Cells”. Methods Mol. Biol., 1850, 289 – 308.
doi: 10.1007/978-1-4939-8730-6_19.
Thoring, L., Dondapati, S. K., Stech, M., Wüstenhagen, D. A., Kubick, S. (2017): „High-yield production of "difficult-to-express" proteins in a continuous exchange cell-free system based on CHO cell lysates”. Sci. Rep., 7(1): 11710.
doi: 10.1038/s41598-017-12188-8.
Thoring, L., Wüstenhagen, D. A., Borowiak, M., Stech, M., Sonnabend, A., Kubick, S. (2016): „Cell-Free Systems Based on CHO Cell Lysates: Optimization Strategies, Synthesis of "Difficult-to-Express" Proteins and Future Perspectives”. PLoS One., 11(9):e0163670.
doi: 10.1371/journal.pone.0163670.
Zemella, A., Thoring, L., Hoffmeister, C., Kubick, S. (2015): „Cell-Free Protein Synthesis: Pros and Cons of Prokaryotic and Eukaryotic Systems”. ChemBioChem, 16, 2420 – 2431.
Brödel, A. K., Wüstenhagen, D. A., Kubick, S. (2015): „Cell-free protein synthesis systems derived from cultured mammalian cells”. Methods Mol. Biol., 1261, 129 – 140.
doi: 10.1007/978-1-4939-2230-7_7.
Stech, M., Quast, R. B., Sachse, R., Schulze, C., Wüstenhagen, D. A., Kubick, S. (2014): „A continuous-exchange cell-free protein synthesis system based on extracts from cultured insect cells”. PLoS One, 9, e96635.
doi: 10.1371/journal.pone.0096635.
Brödel, A. K., Kubick, S. (2014): „Developing cell-free protein synthesis systems: a focus on mammalian cells”. Pharm. Bioprocess., 2(4), 339 – 348.
Brödel, A. K., Sonnabend, A., Kubick, S. (2013): „Cell-free protein expression based on extracts from CHO cells”. Biotechnol. Bioeng., 111, 25 – 36.
Kubick, S., Gerrits, M., Merk, H., Stiege, W., Erdmann, V. A. (2009): „In vitro Synthesis of posttranslationally modified Membrane Proteins”, Chapter 2. In: Membrane Protein Crystallization (Ed.: Larry DeLucas). Current Topics in Membranes, Vol. 63, Elsevier, 25 – 49.
doi: 10.1016/S1063-5823(09)63002-7.